Plasmid_Backbone

Part:BBa_K319033:Design

Designed by: Afnan Azizi   Group: iGEM10_uOttawa   (2010-10-26)


ESV


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2195
    Illegal XbaI site found at 2210
    Illegal SpeI site found at 61
    Illegal PstI site found at 75
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2195
    Illegal SpeI site found at 61
    Illegal PstI site found at 75
    Illegal NotI site found at 68
    Illegal NotI site found at 2201
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2195
    Illegal BglII site found at 35
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found at 61
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2195
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2210
    Illegal SpeI site found at 61
    Illegal PstI site found at 75
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1234


Design Notes

N/A


Source

It was obtained by performing a PCR reaction amplifying the pSB1C3 plasmid and using appropriate overhangs containing an NsiI site to combine with the PstI site and a BglII site to combine with the BamHI site.

References